Open Source Hardware
Hardware Integration
- ESP32-S2 Wrover Module with PSRAM (required for camera operations)
- Camera Module (OV7725) for fluorescence detection
- TFT Display (320×240) with touchscreen interface
- Temperature Control via PWM-controlled heaters and fan
- LED System (TLC59108 driver) for sample illumination
- ADC (TLA202x) for temperature sensing and power monitoring
- USB Mass Storage capability for data export
Core Functionality
Temperature Control System
- PID Controllers for block and lid temperature regulation
- Dual heating zones: sample block and heated lid
- Active cooling via PWM fan control
- Temperature sensors using NTC thermistors with Steinhart-Hart equation
Fluorescence Detection
- 8-channel simultaneous measurement
- Optimized for Cyber green fluorescence (SYBR green) based qPCR
- Absorbtion 497 nanometer blue light, emmision 520 nm green light
- Camera-based detection with custom image processing
- Baseline correction and mask-based region selection
- Real-time data visualization with multi-color plotting
Hardware, Electronics and Software design Files on GitHub here: https://github.com/GaudiLabs/qPocketPCR
All data and images published under an open and public CC BY 4.0 license.